| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1121 | Thermobifida fusca | ATCCAAGCGTGCAAGCCA | TTCGTAGTCGATGGTGCCC | 60.28 | 59.49 | 237 | 79 |
100.00%
|
100.00%
|
| Primer 1122 | Thermobifida fusca | TTCGACCGCAAGCTGGAA | ACTTCTTCCATGCTCGCGT | 59.58 | 59.71 | 170 | 79 |
100.00%
|
100.00%
|
| Primer 1123 | Thermobifida fusca | TGCGCTACAACATGCCCA | ACAGGATTGCCGTTGACGT | 59.97 | 59.93 | 202 | 79 |
100.00%
|
100.00%
|
| Primer 1124 | Thermobifida fusca | AATGGCCTGCTGTGCACT | TGTAGCCGTACTCTGCCCA | 59.88 | 60.30 | 279 | 79 |
100.00%
|
100.00%
|
| Primer 1125 | Thermobifida fusca | AACGACCAGCCGAAACGT | AGCAGTTCGTTCTCGGCA | 59.89 | 59.27 | 212 | 79 |
100.00%
|
100.00%
|
| Primer 1126 | Thermobifida fusca | AACGACCAGCCGAAACGT | AGCAGTTCGTTCTCGGCAA | 59.89 | 59.93 | 212 | 79 |
100.00%
|
100.00%
|
| Primer 1127 | Thermobifida fusca | AACGACCAGCCGAAACGT | CCCACTCCAGCAGTTCGTT | 59.89 | 59.93 | 220 | 79 |
100.00%
|
100.00%
|
| Primer 1128 | Thermobifida fusca | AACGACCAGCCGAAACGT | GCAGTTCGTTCTCGGCAAC | 59.89 | 59.80 | 211 | 79 |
100.00%
|
100.00%
|
| Primer 1129 | Thermobifida fusca | AACGACCAGCCGAAACGT | TCCAGCAGTTCGTTCTCGG | 59.89 | 59.71 | 215 | 79 |
100.00%
|
100.00%
|
| Primer 1130 | Thermobifida fusca | ATGACGCGACCGATAACCC | TTGGTGCAGCACATCCCA | 59.93 | 59.48 | 201 | 79 |
100.00%
|
100.00%
|